High Content Analysis

IDRI is a non-profit organization offering cost effective and efficient, high throughput, high content analysis (HCA) assays for host/pathogen interactions in a state-of-the-art Biosafety level 3 laboratory located in Seattle’s Eastlake neighborhood.

Services
  • High throughput screening for drug candidate identification – we will screen small molecules for activity against pathogen-infected host cells
  • Cytotoxicity – We will test small molecules or vaccine formulations for effects on host cell viability
  • Host cell pathway alteration – We will test small molecules or vaccine formulations for effects on host cell processes
Capabilities

     
  • Fully automated plate and liquid handling
  • High-throughput imaging equipment located in Biosafety level 3 laboratory
  • Magnifications of 4X, 10X, 20X, 40X and 60X
  • White light and/or fluorescence image capture; up to 6 channels per assay
  • Image analysis using MetaXpress software
  • Raw image data provided on an external hard drive upon request
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High-throughput, validated, assays

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Other assays

We can run a number of other assays in low throughput, including:

  • M. tuberculosis-infected human THP-1 or primary cells (PBMCs)
  • Nontuberculous mycobacteria-infected macrophage assays (Mycobacterium avium, Mycobacterium abscessus)
  • Mitochondrial protein synthesis inhibition assay
We can work with collaborators to develop custom assays with their assay of choice.

Deliverables
  • Summary data and raw data will be provided
  • Image data will be provided on an external hard-drive upon request at an additional cost
Contact

For more details regarding IDRI’s HCA services and pricing, please contact hcaservices@idri.org.