Leishmania donovani high content assay
We will measure the intracellular activity of small molecule compounds against human monocytic cells (THP-1) infected with Leishmania donovani. THP-1 cells are differentiated into macrophages and then with L. donovani constitutively expressing DsRed. We expose infected cells to test compounds for 48 hours. Cells are fixed and nuclei stained with DAPI. Cells and parasites are imaged and quantified. Anti-parasitic and cytotoxic activity is measured and reported as IC50 (concentration required to inhibit growth by 50%).
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